UNIT 12.16 Immunohistochemistry

  1. Simon Watkins

Published Online: 1 APR 2009

DOI: 10.1002/0471142956.cy1216s48

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Watkins, S. 2009. Immunohistochemistry. Current Protocols in Cytometry. 48:12.16:12.16.1–12.16.10.

Author Information

  1. University of Pittsburgh Medical School, Pittsburgh, Pennsylvania

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009


Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. Curr. Protocol. Cytom. 48:12.16.1-12.16.10. © 2009 by John Wiley & Sons, Inc.


  • immunofluorescence;
  • microscopy;
  • imaging