UNIT 12.22 Setting Up and Running an Advanced Light Microscopy and Imaging Facility

  1. Carlos Sánchez1,
  2. Ma Ángeles Muñoz1,
  3. Maite Villalba1,
  4. Verónica Labrador1,
  5. F. Javier Díez-Guerra1,2

Published Online: 1 JUL 2011

DOI: 10.1002/0471142956.cy1222s57

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Sánchez, C., Muñoz, M. Á., Villalba, M., Labrador, V. and Díez-Guerra, F. J. 2011. Setting Up and Running an Advanced Light Microscopy and Imaging Facility. Current Protocols in Cytometry. 57:12.22:12.22.1–12.22.21.

Author Information

  1. 1

    Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Madrid, Spain

  2. 2

    Departamento de Biología Molecular. Facultad de Ciencias (UAM), Universidad Autónoma de Madrid, Madrid, Spain

Publication History

  1. Published Online: 1 JUL 2011
  2. Published Print: JUL 2011


During the last twenty years, interest in light microscopy and imaging techniques has grown in various fields, such as molecular and cellular biology, developmental biology, and neurobiology. In addition, the number of scientific articles and journals using these techniques is rapidly increasing. Nowadays, most research institutions require sophisticated microscopy systems to cover their investigation demands. In general, such instruments are too expensive and complex to be purchased and managed by a single laboratory or research group, so they have to be shared with other groups and supervised by specialized personnel. This is the reason why microscopy and imaging facilities are becoming so important at research institutions nowadays. In this unit, we have gathered and presented a number of issues and considerations from our own experience that we hope will be helpful when planning or setting up a new facility. Curr. Protoc. Cytom. 57:12.22.1-12.22.21. © 2011 by John Wiley & Sons, Inc.


  • fluorescence;
  • microscopy;
  • confocal;
  • facility