UNIT 12.23 Photoactivation and Imaging of Optical Highlighter Fluorescent Proteins

  1. George H. Patterson

Published Online: 1 JUL 2011

DOI: 10.1002/0471142956.cy1223s57

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Patterson, G. H. 2011. Photoactivation and Imaging of Optical Highlighter Fluorescent Proteins. Current Protocols in Cytometry. 57:12.23:12.23.1–12.23.12.

Author Information

  1. Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 JUL 2011
  2. Published Print: JUL 2011


A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be “turned on” by photoactivation. This unit discusses technical aspects for the general use of photoactivatable fluorescent proteins and introduces some specific applications in the concluding remarks. Curr. Protoc. Cytom. 57:12.23.1-12.23.12. © 2011 by John Wiley & Sons, Inc.


  • photoactivatable;
  • fluorescent protein;
  • microscopy