Unit

UNIT 12.28 Live Imaging of the Lung

  1. Emily E. Thornton1,
  2. Matthew F. Krummel1,
  3. Mark R. Looney2,3

Published Online: 1 APR 2012

DOI: 10.1002/0471142956.cy1228s60

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Thornton, E. E., Krummel, M. F. and Looney, M. R. 2012. Live Imaging of the Lung. Current Protocols in Cytometry. 60:12.28:12.28.1–12.28.12.

Author Information

  1. 1

    Department of Pathology, University of California, San Francisco, California

  2. 2

    Department of Medicine, University of California, San Francisco, California

  3. 3

    Department of Laboratory Medicine, University of California, San Francisco, California

Publication History

  1. Published Online: 1 APR 2012
  2. Published Print: APR 2012

Abstract

Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung. Curr. Protoc. Cytom. 60:12.28.1-12.28.12. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • intravital imaging;
  • lung imaging;
  • lung slices;
  • two-photon microscopy