Unit

UNIT 12.35 The Application of KillerRed for Acute Protein Inactivation in Living Cells

  1. Timothy S. Jarvela,
  2. Adam D. Linstedt

Published Online: 1 JUL 2014

DOI: 10.1002/0471142956.cy1235s69

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Jarvela, T. S. and Linstedt, A. D. 2014. The Application of KillerRed for Acute Protein Inactivation in Living Cells. Current Protocols in Cytometry. 69:12.35:12.35.1–12.35.10.

Author Information

  1. Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania

Publication History

  1. Published Online: 1 JUL 2014

Abstract

Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live-cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivation (CALI) uses genetic encoding for specificity and light for acute inactivation that can also be spatially restricted. This unit provides protocols for setting up and carrying out properly controlled KillerRed experiments during live-cell imaging of cultured cells. Curr. Protoc. Cytom. 69:12.35.1-12.35.10. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • KillerRed;
  • CALI;
  • acute inactivation;
  • protein inactivation;
  • ROS