UNIT 13.2 Microsphere Surface Protein Determination Using Flow Cytometry
Published Online: 1 NOV 2005
Copyright © 2004 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Woods, T. A., Graves, S. W. and Nolan, J. P. 2005. Microsphere Surface Protein Determination Using Flow Cytometry. Current Protocols in Cytometry. 34:13.2:13.2.1–13.2.13.
- Published Online: 1 NOV 2005
- Published Print: OCT 2005
This unit describes an extrinsic staining protocol using the amine-reactive CBQCA dye to measure the amount of protein on the surface of a microsphere. This approach is novel in that it allows microspheres bearing proteins without known binding partners to be accurately quantified on a flow cytometer.
- flow cytometry;
- surface chemistry;