UNIT 13.2 Microsphere Surface Protein Determination Using Flow Cytometry

  1. Travis A. Woods1,
  2. Steven W. Graves1,
  3. John P. Nolan2

Published Online: 1 NOV 2005

DOI: 10.1002/0471142956.cy1302s34

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Woods, T. A., Graves, S. W. and Nolan, J. P. 2005. Microsphere Surface Protein Determination Using Flow Cytometry. Current Protocols in Cytometry. 34:13.2:13.2.1–13.2.13.

Author Information

  1. 1

    Los Alamos National Laboratory, Los Alamos, New Mexico

  2. 2

    La Jolla Bioengineering Institute, La Jolla, California

Publication History

  1. Published Online: 1 NOV 2005
  2. Published Print: OCT 2005


This unit describes an extrinsic staining protocol using the amine-reactive CBQCA dye to measure the amount of protein on the surface of a microsphere. This approach is novel in that it allows microspheres bearing proteins without known binding partners to be accurately quantified on a flow cytometer.


  • flow cytometry;
  • surface chemistry;
  • protein;
  • microspheres