Unit

UNIT 13.3 Use of Microsphere-Supported Phospholipid Membranes for Analysis of Protein-Lipid Interactions

  1. Gary E. Gilbert

Published Online: 1 NOV 2005

DOI: 10.1002/0471142956.cy1303s34

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Gilbert, G. E. 2005. Use of Microsphere-Supported Phospholipid Membranes for Analysis of Protein-Lipid Interactions. Current Protocols in Cytometry. 34:13.3:13.3.1–13.3.10.

Author Information

  1. Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2005
  2. Published Print: OCT 2005

Abstract

Many proteins bind to phospholipid membranes in order to gain or modify function. As an example, blood coagulation proteins must bind to phosphatidylserine-containing membranes in order to gain procoagulant activity. The flow cytometry approaches described in this unit provide versatile assays for equilibrium and kinetic binding studies to measure essential membrane-binding properties. These assays are rapid, require only small quantities of the protein of interest, and can evaluate binding interactions in complex, physiological mixtures, including blood plasma and tissue culture media.

Keywords:

  • lipospheres;
  • phosphatidylserine;
  • phosphatidylcholine;
  • factor VIII;
  • factor V;
  • factor IX;
  • factor X;
  • prothrombin;
  • lactadherin;
  • annexin V