UNIT 13.8 Reagents and Instruments for Multiplexed Analysis Using Microparticles
Published Online: 1 AUG 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Nolan, J. P., Yang, L. and van der Heyde, H. C. 2006. Reagents and Instruments for Multiplexed Analysis Using Microparticles. Current Protocols in Cytometry. 37:13.8:13.8.1–13.8.10.
- Published Online: 1 AUG 2006
- Published Print: JUL 2006
Multiplexed molecular analysis by means of flow cytometry using optically encoded microspheres is a rapidly expanding application that has its roots in the earliest days of flow cytometry. The approach is driven by increasing demand for analytical methods to measure large numbers of biomolecules quantitatively and sensitively in small volumes of sample. Encoded microspheres and flow cytometry have been employed for a wide range of multiplexed molecular analysis, and detailed protocols for many of these have been developed. The goal of this unit is to provide an overview of the concepts, instruments, and reagents that enable these assays.
- flow cytometry, proteomic, genomic, quantitation, molecular