UNIT 13.8 Reagents and Instruments for Multiplexed Analysis Using Microparticles

  1. John P. Nolan,
  2. Loretta Yang,
  3. Henri C. van der Heyde

Published Online: 1 AUG 2006

DOI: 10.1002/0471142956.cy1308s37

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Nolan, J. P., Yang, L. and van der Heyde, H. C. 2006. Reagents and Instruments for Multiplexed Analysis Using Microparticles. Current Protocols in Cytometry. 37:13.8:13.8.1–13.8.10.

Author Information

  1. La Jolla Bioengineering Institute, La Jolla, California

Publication History

  1. Published Online: 1 AUG 2006
  2. Published Print: JUL 2006


Multiplexed molecular analysis by means of flow cytometry using optically encoded microspheres is a rapidly expanding application that has its roots in the earliest days of flow cytometry. The approach is driven by increasing demand for analytical methods to measure large numbers of biomolecules quantitatively and sensitively in small volumes of sample. Encoded microspheres and flow cytometry have been employed for a wide range of multiplexed molecular analysis, and detailed protocols for many of these have been developed. The goal of this unit is to provide an overview of the concepts, instruments, and reagents that enable these assays.


  • flow cytometry, proteomic, genomic, quantitation, molecular