APPENDIX 3B Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 MAY 2006

DOI: 10.1002/0471142956.cya03bs36

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Phelan, M. C. 2006. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Cytometry. 36:3B:A.3B.1–A.3B.18.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 MAY 2006
  2. Published Print: APR 2006


This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.


  • mammalian cells;
  • cell culture;
  • trypsinizing;
  • passaging;
  • hemacytometer