Appendix

APPENDIX 3E Techniques for Bacterial Cell Culture: Media Preparation and Bacteriological Tools

  1. Karen Lech,
  2. Roger Brent

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cya03es11

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Lech, K. and Brent, R. 2001. Techniques for Bacterial Cell Culture: Media Preparation and Bacteriological Tools. Current Protocols in Cytometry. 11:3E:A.3E.1–A.3E.8.

Author Information

  1. Massachusetts General Hospital and Harvard, Medical School Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2000

Abstract

Basic information for the culture of bacteria is presented in this appendix, using Escherichia coli as a representative organism. The techniques and recipes should be readily transferrable. Specifics are given for liquid and solid media, rich and minimal media, stab agar, as well as a description of the tools required to inoculate and culture bacteria.