APPENDIX 3F Growing Bacteria in Liquid Media
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Lech, K. and Brent, R. 2001. Growing Bacteria in Liquid Media. Current Protocols in Cytometry. 12:3F:A.3F.1–A.3F.2.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.