APPENDIX 3F Growing Bacteria in Liquid Media

  1. Karen Lech,
  2. Roger Brent

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cya03fs12

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Lech, K. and Brent, R. 2001. Growing Bacteria in Liquid Media. Current Protocols in Cytometry. 12:3F:A.3F.1–A.3F.2.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000


The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.