APPENDIX 3G Growing Bacteria on Solid Media

  1. Karen Lech,
  2. Roger Brent

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cya03gs12

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Lech, K. and Brent, R. 2001. Growing Bacteria on Solid Media. Current Protocols in Cytometry. 12:3G:A.3G.1–A.3G.5.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000


Detailed protocols are provided for titering and isolating bacterial colonies by serial dilutions, or alternatively by streaking or spreading a plate. Support protocols describe replica plating as well as methods for storing strains as agar stabs or frozen glycerol stocks.