APPENDIX 3I Production of Polyclonal Antisera

  1. Helen M. Cooper1,
  2. Yvonne Paterson2

Published Online: 1 AUG 2006

DOI: 10.1002/0471142956.cya03is37

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Cooper, H. M. and Paterson, Y. 2006. Production of Polyclonal Antisera. Current Protocols in Cytometry. 3:3I.

Author Information

  1. 1

    Ludwig Institute for Cancer Research, Melbourne, Australia

  2. 2

    University of Pennsylvania, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 AUG 2006
  2. Published Print: JUL 2006

This is not the most recent version of the article. View current version (1 JAN 2008)


Although the advances offered by the development of monoclonal antibody techniques have revolutionized the specificity, uniformity, and quantity of antibodies, there remain many circumstances in which polyclonal antibodies are more desirable than monoclonal antibodies. Production of polyclonal antisera takes less time and effort than production of monoclonal antibodies, requires relatively simple and readily available equipment, and produces reagents that can be used for immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assays (ELISAs). The Basic Protocol and Alternate Protocol in this unit describe the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters. The Support Protocol presents a method for preparing serum from blood.