Appendix

APPENDIX 3J Production of Monoclonal Antibodies

  1. Wayne M. Yokoyama

Published Online: 1 AUG 2006

DOI: 10.1002/0471142956.cya03js37

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Yokoyama, W. M. 2006. Production of Monoclonal Antibodies. Current Protocols in Cytometry. 37:3J:A.3J.1–A.3J.17.

Author Information

  1. University of California School of Medicine, San Francisco, California

Publication History

  1. Published Online: 1 AUG 2006
  2. Published Print: JUL 2006

Abstract

This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium (thymocyte-conditioned medium).