Unit

UNIT 1.1 Basic Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0101s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Phelan, M. C. 2001. Basic Techniques for Mammalian Cell Tissue Culture. Current Protocols in Cell Biology. 1:1.1.

Author Information

  1. Greenwood Genetic Center, Greenwood, South Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

This is not the most recent version of the article. View current version (2 MAR 2015)

Abstract

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.