Unit

UNIT 1.1 Basic Techniques in Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 SEP 2007

DOI: 10.1002/0471143030.cb0101s36

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Phelan, M. C. 2007. Basic Techniques in Mammalian Cell Tissue Culture. Current Protocols in Cell Biology. 36:1.1:1.1.1–1.1.18.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 SEP 2007
  2. Published Print: SEP 2007

This is not the most recent version of the article. View current version (2 MAR 2015)

Abstract

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. Curr. Protoc. Cell Biol. 36:1.1.1-1.1.18. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • mammalian cells;
  • tissue culture;
  • aseptic technique;
  • medium;
  • passaging cells;
  • freezing cells