UNIT 1.1 Basic Techniques in Mammalian Cell Tissue Culture
Published Online: 1 SEP 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Phelan, M. C. 2007. Basic Techniques in Mammalian Cell Tissue Culture. Current Protocols in Cell Biology. 36:1.1:1.1.1–1.1.18.
- Published Online: 1 SEP 2007
- Published Print: SEP 2007
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Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. Curr. Protoc. Cell Biol. 36:1.1.1-1.1.18. © 2007 by John Wiley & Sons, Inc.
- mammalian cells;
- tissue culture;
- aseptic technique;
- passaging cells;
- freezing cells