UNIT 1.2 Media for Culture of Mammalian Cells

  1. J. Denry Sato1,
  2. Mikio Kan2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0102s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Sato, J. D. and Kan, M. 2001. Media for Culture of Mammalian Cells. Current Protocols in Cell Biology. 00:1.2:1.2.1–1.2.15.

Author Information

  1. 1

    Adirondack Biomedical Research Institute, Lake Placid, New York

  2. 2

    Texas A&M University, Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


When mammalian cells are cultured in vitro, the investigator is attempting to reproduce the physiological environment in order to maintain and analyze normal functions and responses. The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This unit provides guidelines for design of serum-containing and serum-free media, selective andspecialty media, and media for growth under special conditions such as soft-agar growth.