UNIT 1.3 Aseptic Technique for Cell Culture

  1. Rosalie J. Coté

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0103s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Coté, R. J. 2001. Aseptic Technique for Cell Culture. Current Protocols in Cell Biology. 00:1.3:1.3.1–1.3.10.

Author Information

  1. Becton Dickinson Microbiology Systems, Sparks, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


This unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch-all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. In reality, aseptic technique encompasses all aspects of environmental control, personal hygiene, equipment and media sterilization, and associated quality control procedures needed to ensure that a procedure is, indeed, performed with aseptic, noncontaminating technique. Although cell culture can theoretically be carried out on an open bench in a low-traffic area, most cell culture work is carried out using a horizontal laminar-flow clean bench or a vertical laminar-flow biosafety cabinet. Both are described here.