UNIT 2.1 Establishment of Fibroblast Cultures

  1. Akira Takashima

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0201s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Takashima, A. 2001. Establishment of Fibroblast Cultures. Current Protocols in Cell Biology. 00:2.1:2.1.1–2.1.12.

Author Information

  1. University of Texas Southwest Medical Center, Dallas, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


Fibroblasts are fairly easily isolated from a variety of tissues, in this case skin. The cells are fast growing and can be rapidly expanded from small samples. They are suitable for behavioral, functional, biochemical, and genomic studies. Skin fibroblast cultures are started from explants in which the epidermis is enzymatically removed to prevent contamination of the fibroblasts with epidermal cells. Alternatively, the dermis can be enzymatically dissociated to provide a suspension of fibroblasts.