Unit

UNIT 2.2 Preparation and Culture of Human Lymphocytes

  1. William E. Biddison

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0202s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Biddison, W. E. 2001. Preparation and Culture of Human Lymphocytes. Current Protocols in Cell Biology. 00:2.2:2.2.1–2.2.13.

Author Information

  1. National Institute of Neurological Disorders and Stroke/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

This unit presents protocols for preparation of lymphocytes from peripheral (whole) blood or leucopherisis samples, first by differential centrifugation in Ficoll-Hypaque to separate them from erythrocytes and granulocytes. Repeated centrifugation removes the plasma and platelets. Monocyte/macrophage cells and dendritic cells can be purified from the T and B cell population by exploiting their adhesion to serum-coated plates in the presence of recombinant IL-3. Different subpopulations of lymphocytes can be isolated by specific antibodies bound to magnetic beads.