UNIT 2.2 Preparation and Culture of Human Lymphocytes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Biddison, W. E. 2001. Preparation and Culture of Human Lymphocytes. Current Protocols in Cell Biology. 00:2.2:2.2.1–2.2.13.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
This unit presents protocols for preparation of lymphocytes from peripheral (whole) blood or leucopherisis samples, first by differential centrifugation in Ficoll-Hypaque to separate them from erythrocytes and granulocytes. Repeated centrifugation removes the plasma and platelets. Monocyte/macrophage cells and dendritic cells can be purified from the T and B cell population by exploiting their adhesion to serum-coated plates in the presence of recombinant IL-3. Different subpopulations of lymphocytes can be isolated by specific antibodies bound to magnetic beads.