UNIT 2.3 Preparation of Endothelial Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Kleinman, H. K. and Cid, M. C. 2001. Preparation of Endothelial Cells. Current Protocols in Cell Biology. 00:2.3:2.3.1–2.3.6.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
Endothelial cells, which line blood vessels, can be prepared from a variety of tissues. They are frequently prepared from the umbilical vein, which is relatively easy to obtain. The procedure is clearly described and provides a large population of highly purified endothelial cells. There are also methods for obtaining endothelial cells from other tissues such as fat, skin, and mucosa. These methods require special care and generate smaller populations of cells.