UNIT 2.3 Preparation of Endothelial Cells

  1. Hynda K. Kleinman1,
  2. Maria C. Cid2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0203s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Kleinman, H. K. and Cid, M. C. 2001. Preparation of Endothelial Cells. Current Protocols in Cell Biology. 00:2.3:2.3.1–2.3.6.

Author Information

  1. 1

    National Institute of Dental Research/NIH, Bethesda, Maryland

  2. 2

    Hospital Clinic i Provincia, Barcelona, Spain

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


Endothelial cells, which line blood vessels, can be prepared from a variety of tissues. They are frequently prepared from the umbilical vein, which is relatively easy to obtain. The procedure is clearly described and provides a large population of highly purified endothelial cells. There are also methods for obtaining endothelial cells from other tissues such as fat, skin, and mucosa. These methods require special care and generate smaller populations of cells.