Unit

UNIT 2.4 Generation of Continuously Growing B Cell Lines by Epstein-Barr Virus Transformation

  1. William E. Biddison

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0204s01

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Biddison, W. E. 2001. Generation of Continuously Growing B Cell Lines by Epstein-Barr Virus Transformation. Current Protocols in Cell Biology. 1:2.4:2.4.1–2.4.3.

Author Information

  1. National Institute of Neurological Disorders and Stroke, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1999

Abstract

Primary cultures of cells tend to have a limited life span, which in turn limits the availability of a consistent population of cells to study. In this unit, Epstein-Barr virus produced by a marmoset cell line is used to transform B cells into a continuously growing cell line that produces no virus. These cell lines can be used as a constant source of cells for further study.