UNIT 2.6 Preparation of Human Epidermal Keratinocyte Cultures
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Yamada, S. S. 2004. Preparation of Human Epidermal Keratinocyte Cultures. Current Protocols in Cell Biology. 21:2.6:2.6.1–2.6.5.
- Published Online: 1 FEB 2004
- Published Print: DEC 2003
Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.
- keratinocyte cultures;
- establishment of epidermal keratinocyte culture keratinocytes;
- human keratinocytes;
- murine keratinocytes;
- morphological features