UNIT 2.6 Preparation of Human Epidermal Keratinocyte Cultures

  1. Susan S. Yamada

Published Online: 1 FEB 2004

DOI: 10.1002/0471143030.cb0206s21

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Yamada, S. S. 2004. Preparation of Human Epidermal Keratinocyte Cultures. Current Protocols in Cell Biology. 21:2.6:2.6.1–2.6.5.

Author Information

  1. National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: DEC 2003


Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.


  • keratinocyte cultures;
  • establishment of epidermal keratinocyte culture keratinocytes;
  • human keratinocytes;
  • murine keratinocytes;
  • morphological features