UNIT 3.1 Overview of Cell Fractionation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Castle, J. D. 2001. Overview of Cell Fractionation. Current Protocols in Cell Biology. 00:3.1:3.1.1–3.1.9.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
This is not the most recent version of the article. View current version (1 DEC 2015)
Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials for in vitro reconstitution studies This overview discusses the basic principles of centrifugation, the instruments available, choice of media, evaluation of fractionation, and procedure optimization.