Unit

UNIT 3.1 Overview of Cell Fractionation

  1. J. David Castle

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0301s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Castle, J. D. 2001. Overview of Cell Fractionation. Current Protocols in Cell Biology. 00:3.1:3.1.1–3.1.9.

Author Information

  1. University of Virginia, Charlottesville, Virginia

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials for in vitro reconstitution studies This overview discusses the basic principles of centrifugation, the instruments available, choice of media, evaluation of fractionation, and procedure optimization.