Unit

UNIT 3.7 Overview of Subcellular Fractionation Procedures for the Yeast Saccharomyces cerevisiae

  1. Stephanie E. Rieder,
  2. Scott D. Emr

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0307s07

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Rieder, S. E. and Emr, S. D. 2001. Overview of Subcellular Fractionation Procedures for the Yeast Saccharomyces cerevisiae. Current Protocols in Cell Biology. 7:3.7:3.7.1–3.7.25.

Author Information

  1. University of California, San Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

This unit provides an overview of centrifugation-based fractionation procedures adapted for the yeast Saccharomyces cerevisiae. The goals, merits, limitations, and critical parameters of are discussed in order to facilitate the development of subcellular fractionation strategies. Topics include yeast cell lysate preparation, differential velocity centrifugation, density gradient centrifugation, and the analysis of subcellular fractions.