Unit

UNIT 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation

  1. Dawn L. Brasaemle1,
  2. Nathan E. Wolins2

Published Online: 1 JAN 2006

DOI: 10.1002/0471143030.cb0315s29

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Brasaemle, D. L. and Wolins, N. E. 2006. Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation. Current Protocols in Cell Biology. 29:3.15:3.15.1–3.15.12.

Author Information

  1. 1

    Rutgers, The State University of New Jersey, New Brunswick, New Jersey

  2. 2

    Washington University of St. Louis School of Medicine, St. Louis, Missouri

Publication History

  1. Published Online: 1 JAN 2006
  2. Published Print: DEC 2005

Abstract

Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.

Keywords:

  • Cell cultures;
  • mammalian;
  • preparation of lipid droplets from centrifugation;
  • density-gradient centrifugation;
  • isolation of lipid droplets