UNIT 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation
Published Online: 1 JAN 2006
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Brasaemle, D. L. and Wolins, N. E. 2006. Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation. Current Protocols in Cell Biology. 29:3.15:3.15.1–3.15.12.
- Published Online: 1 JAN 2006
- Published Print: DEC 2005
Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.
- Cell cultures;
- preparation of lipid droplets from centrifugation;
- density-gradient centrifugation;
- isolation of lipid droplets