Unit

UNIT 3.18 Isolation of Zymogen Granules from Rat Pancreas

  1. Michael J. Rindler

Published Online: 1 JAN 2006

DOI: 10.1002/0471143030.cb0318s29

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Rindler, M. J. 2006. Isolation of Zymogen Granules from Rat Pancreas. Current Protocols in Cell Biology. 29:3.18:3.18.1–3.18.16.

Author Information

  1. New York University School of Medicine, New York, New York

Publication History

  1. Published Online: 1 JAN 2006
  2. Published Print: DEC 2005

Abstract

This unit describes methods for preparing zymogen granules from rat pancreas. Zymogen granules are storage organelles in pancreatic acinar cells containing digestive enzymes that are released into the pancreatic duct. The protocols in this unit take advantage of the large size (up to 1 µm diameter) and high density (>1.20 g/cm3 on sucrose gradients) of the granules as compared to other cellular organelles. They use a combination of differential sedimentation and density gradient separation to accomplish the purification. Similar procedures can be used to isolate zymogen granules from mouse pancreas and canine pancreas. A protocol for preparing zymogen granules from dog pancreas is also included.

Keywords:

  • pancreas;
  • zymogen granules;
  • secretory granules;
  • amylase;
  • exocrine;
  • rat