UNIT 3.18 Isolation of Zymogen Granules from Rat Pancreas
Published Online: 1 JAN 2006
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Rindler, M. J. 2006. Isolation of Zymogen Granules from Rat Pancreas. Current Protocols in Cell Biology. 29:3.18:3.18.1–3.18.16.
- Published Online: 1 JAN 2006
- Published Print: DEC 2005
This unit describes methods for preparing zymogen granules from rat pancreas. Zymogen granules are storage organelles in pancreatic acinar cells containing digestive enzymes that are released into the pancreatic duct. The protocols in this unit take advantage of the large size (up to 1 µm diameter) and high density (>1.20 g/cm3 on sucrose gradients) of the granules as compared to other cellular organelles. They use a combination of differential sedimentation and density gradient separation to accomplish the purification. Similar procedures can be used to isolate zymogen granules from mouse pancreas and canine pancreas. A protocol for preparing zymogen granules from dog pancreas is also included.
- zymogen granules;
- secretory granules;