Unit

UNIT 3.24 Isolation of T-Tubules from Skeletal Muscle

  1. Antonio Zorzano,
  2. Marta Camps

Published Online: 1 JUL 2006

DOI: 10.1002/0471143030.cb0324s31

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Zorzano, A. and Camps, M. 2006. Isolation of T-Tubules from Skeletal Muscle. Current Protocols in Cell Biology. 31:3.24:3.24.1–3.24.14.

Author Information

  1. Departament de Bioquimica i Biologia, Molecular, Universitat de Barcelona, and Institute for Research in Biomedicine, Barcelona Science Park, Barcelona, Spain

Publication History

  1. Published Online: 1 JUL 2006
  2. Published Print: JUN 2006

Abstract

The transverse tubules (T-tubules) of mammalian cardiac and skeletal muscles are invaginations of the sarcolemma. They play a crucial role in excitation-contraction coupling as well as in intracellular signaling and in regulation of glucose transport. The biochemical purification of T-tubule membranes is a difficult task, and membrane fractions enriched in transverse tubules are usually contaminated with other cell-surface and intracellular membranes. This unit includes methods that permit the isolation and purification of T-tubules from skeletal muscle.

Keywords:

  • T-tubules;
  • transverse tubules;
  • vesicle immunoisolation;
  • wheat germ agglutination;
  • skeletal muscle;
  • dihydropyridine receptors;
  • tt28 protein