Unit

UNIT 3.25 Isolation of Myelin

  1. Jorge N. Larocca,
  2. Williams T. Norton

Published Online: 1 JAN 2007

DOI: 10.1002/0471143030.cb0325s33

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Larocca, J. N. and Norton, W. T. 2007. Isolation of Myelin. Current Protocols in Cell Biology. 33:3.25:3.25.1–3.25.19.

Author Information

  1. Albert Einstein College of Medicine, Yeshiva University, Bronx, New York

Publication History

  1. Published Online: 1 JAN 2007
  2. Published Print: DEC 2006

Abstract

The methods used to prepare myelin involve homogenization of the tissue in isotonic sucrose solution, followed by the isolation of myelin membranes by a series of steps that include density gradient centrifugation and differential centrifugation. Homogenization of nervous tissue in isotonic sucrose causes the myelin sheath to peel from the axon and form relatively large myelin vesicles. The large size of the myelin vesicles, together with the fact that myelin membrane has a lower density than other biological membranes, make differential centrifugation and density gradient centrifugation the main tools for the isolation of this membrane. Three protocols are outlined in this unit: isolation of a highly-purified myelin fraction from the central nervous system (CNS); separation of a highly-purified CNS myelin fraction into subfractions of different densities; and isolation of myelin from the peripheral nervous system (PNS).

Keywords:

  • CNS myelin;
  • PNS myelin;
  • CNPase;
  • oligodendrocytes;
  • Schwann cell;
  • brain;
  • sciatic nerve