Unit

UNIT 3.26 Isolation of Renal Brush Borders

  1. D. James Morré1,
  2. Timothy Hammond2

Published Online: 1 MAR 2007

DOI: 10.1002/0471143030.cb0326s34

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Morré, D. J. and Hammond, T. 2007. Isolation of Renal Brush Borders. Current Protocols in Cell Biology. 33:3.26:3.26.1–3.26.14.

Author Information

  1. 1

    Purdue University, West Lafayette, Indiana

  2. 2

    Tulane University School of Medicine and Southeast Louisianna Veterans Health Care System, New Orleans, Louisianna

Publication History

  1. Published Online: 1 MAR 2007
  2. Published Print: MAR 2007

Abstract

Methods are described to isolate intact brush borders and brush border membranes from renal cell homogenates. A rapid method yields sealed vesicles that reconstitute renal brush border transport. In one variation of this protocol, 10 to 20 mM CaCl2 or MgCl2 is added to aggregate non-brush border structures for subsequent removal by centrifugation. For analytical studies, guidance is provided for subsequent purification steps including preparative free-flow and aqueous two-phase partition. Marker enzymes and morphological parameters are included for assessment of yield and fraction purity.

Keywords:

  • kidney;
  • brush border;
  • isolation;
  • aqueous two phase