UNIT 3.26 Isolation of Renal Brush Borders
Published Online: 1 MAR 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Morré, D. J. and Hammond, T. 2007. Isolation of Renal Brush Borders. Current Protocols in Cell Biology. 33:3.26:3.26.1–3.26.14.
- Published Online: 1 MAR 2007
- Published Print: MAR 2007
Methods are described to isolate intact brush borders and brush border membranes from renal cell homogenates. A rapid method yields sealed vesicles that reconstitute renal brush border transport. In one variation of this protocol, 10 to 20 mM CaCl2 or MgCl2 is added to aggregate non-brush border structures for subsequent removal by centrifugation. For analytical studies, guidance is provided for subsequent purification steps including preparative free-flow and aqueous two-phase partition. Marker enzymes and morphological parameters are included for assessment of yield and fraction purity.
- brush border;
- aqueous two phase