Unit

UNIT 3.32 Isolation of Dense Core Secretory Vesicles from Pancreatic Endocrine Cells by Differential and Density Gradient Centrifugation

  1. John C. Hutton,
  2. Randall Wong,
  3. Howard W. Davidson

Published Online: 1 MAR 2009

DOI: 10.1002/0471143030.cb0332s42

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Hutton, J. C., Wong, R. and Davidson, H. W. 2009. Isolation of Dense Core Secretory Vesicles from Pancreatic Endocrine Cells by Differential and Density Gradient Centrifugation. Current Protocols in Cell Biology. 42:3.32:3.32.1–3.32.20.

Author Information

  1. Barbara Davis Center for Childhood Diabetes, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado

Publication History

  1. Published Online: 1 MAR 2009
  2. Published Print: MAR 2009

Abstract

Methods are presented for the separation of dense core secretory vesicles from insulin-secreting tissues (insulin granules) based on a combination of differential and density gradient centrifugation on various media. Emphasis is given to the use of transplantable tumors, tissue culture cell lines, and pancreatic islets as a tissue source. Curr. Protoc. Cell Biol. 42:3.32.1-3.32.20. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • insulin granules;
  • DCV;
  • insulinoma;
  • Min6;
  • INS1;
  • homogenization;
  • centrifugation