UNIT 3.32 Isolation of Dense Core Secretory Vesicles from Pancreatic Endocrine Cells by Differential and Density Gradient Centrifugation
Published Online: 1 MAR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Hutton, J. C., Wong, R. and Davidson, H. W. 2009. Isolation of Dense Core Secretory Vesicles from Pancreatic Endocrine Cells by Differential and Density Gradient Centrifugation. Current Protocols in Cell Biology. 42:3.32:3.32.1–3.32.20.
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
Methods are presented for the separation of dense core secretory vesicles from insulin-secreting tissues (insulin granules) based on a combination of differential and density gradient centrifugation on various media. Emphasis is given to the use of transplantable tumors, tissue culture cell lines, and pancreatic islets as a tissue source. Curr. Protoc. Cell Biol. 42:3.32.1-3.32.20. © 2009 by John Wiley & Sons, Inc.
- insulin granules;