Unit

UNIT 3.36 Isolation of Nucleoli

  1. Sabine Hacot1,
  2. Yohann Coute2,
  3. Stéphane Belin1,
  4. Marie Alexandra Albaret1,
  5. Hichem C. Mertani1,
  6. Jean-Charles Sanchez3,
  7. Manuel Rosa-Calatrava1,
  8. Jean-Jacques Diaz1

Published Online: 1 JUN 2010

DOI: 10.1002/0471143030.cb0336s47

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Hacot, S., Coute, Y., Belin, S., Albaret, M. A., Mertani, H. C., Sanchez, J.-C., Rosa-Calatrava, M. and Diaz, J.-J. 2010. Isolation of Nucleoli. Current Protocols in Cell Biology. 47:3.36:3.36.1–3.36.10.

Author Information

  1. 1

    Université de Lyon, Lyon, France

  2. 2

    CEA, DSV, iRTSV, Laboratoire d'Etude de la Dynamique des Protéomes, Grenoble, France and Université Joseph Fourier, Grenoble, France

  3. 3

    Biomedical Proteomics Research Group, Département de Biologie Structurale et Bioinformatique, Centre Médical Universitaire, Geneva, Switzerland

Publication History

  1. Published Online: 1 JUN 2010
  2. Published Print: JUN 2010

Abstract

Nucleoli are now recognized as multi-functional nuclear domains involved in several fundamental cell processes such as ribosome biogenesis, regulation of the assembly of non-ribosomal ribonucleoprotein complexes, tRNA maturation, sequestration of protein, viral infection, and cellular ageing. Extensive proteomic analyses of these nucleolar domains after their purification have contributed to the description of their multiple biological functions. Because nucleoli are the largest and densest nuclear structures, they are easily amenable to purification from nuclei of cultured animal cells using the protocol described in this unit. Curr. Protoc. Cell Biol. 47:3.36.1-3.36.10. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • nuclear domain;
  • nucleoli;
  • cell fractionation