UNIT 3.36 Isolation of Nucleoli
Published Online: 1 JUN 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Hacot, S., Coute, Y., Belin, S., Albaret, M. A., Mertani, H. C., Sanchez, J.-C., Rosa-Calatrava, M. and Diaz, J.-J. 2010. Isolation of Nucleoli. Current Protocols in Cell Biology. 47:3.36:3.36.1–3.36.10.
- Published Online: 1 JUN 2010
- Published Print: JUN 2010
Nucleoli are now recognized as multi-functional nuclear domains involved in several fundamental cell processes such as ribosome biogenesis, regulation of the assembly of non-ribosomal ribonucleoprotein complexes, tRNA maturation, sequestration of protein, viral infection, and cellular ageing. Extensive proteomic analyses of these nucleolar domains after their purification have contributed to the description of their multiple biological functions. Because nucleoli are the largest and densest nuclear structures, they are easily amenable to purification from nuclei of cultured animal cells using the protocol described in this unit. Curr. Protoc. Cell Biol. 47:3.36.1-3.36.10. © 2010 by John Wiley & Sons, Inc.
- nuclear domain;
- cell fractionation