UNIT 4.3 Immunofluorescence Staining
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Donaldson, J. G. 2001. Immunofluorescence Staining. Current Protocols in Cell Biology. 00:4.3:4.3.1–4.3.6.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
This is not the most recent version of the article. View current version (1 DEC 2015)
This unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.