UNIT 4.3 Immunofluorescence Staining

  1. Julie G. Donaldson

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0403s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Donaldson, J. G. 2001. Immunofluorescence Staining. Current Protocols in Cell Biology. 00:4.3:4.3.1–4.3.6.

Author Information

  1. National Heart, Lung, and Blood Institute/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

This is not the most recent version of the article. View current version (1 DEC 2015)


This unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.