Unit

UNIT 4.4 Fluorescent Staining of Subcellular Organelles: ER, Golgi Complex, and Mitochondria

  1. Mark Terasaki (ER and mitochondria)1,
  2. Leslie Loew (mitochondria)1,
  3. Jennifer Lippincott-Schwartz2,
  4. Kristien Zaal (Golgi complex)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0404s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Terasaki, M., Loew, L., Lippincott-Schwartz, J. and Zaal, K. 2001. Fluorescent Staining of Subcellular Organelles: ER, Golgi Complex, and Mitochondria. Current Protocols in Cell Biology. 00:4.4:4.4.1–4.4.18.

Author Information

  1. 1

    University of Connecticut Health Center, Farmington, Connecticut

  2. 2

    National Institute of Child Health and Human Development/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1999

Abstract

The ability to distinguish and identify specific subcellular compartments is essential to understanding organelle function, biogenesis, and maintenance within cells and to defining protein trafficking pathways. Fluorescent dyes and/or fluorescently labeled lipid derivatives can be used to identify ER, Golgi complex, and mitochondria. Specific conditions for labeling each of these compartments are described.