UNIT 4.6 Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Brown, W. J. 2001. Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues. Current Protocols in Cell Biology. 1:4.6:4.6.1–4.6.17.
- Published Online: 1 MAY 2001
- Published Print: JAN 1999
This carefully written unit describes two methods for using the immunoperoxidase reaction to localize antigens at the electron microscope level; one for adherent cultured cells and one for tissue sections. The reaction conditions are first optimized at the light microscope level and then adapted for EM level observation. These methods allow for reliable detection of antigens at the cell surface, within the cell, and especially in membrane bounded organelles. Embedding and staining procedures are also optimized for sample visualization.