UNIT 4.8 Correlative Video Light/Electron Microscopy
Published Online: 1 AUG 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Polishchuk, R. S. and Mironov, A. A. 2001. Correlative Video Light/Electron Microscopy. Current Protocols in Cell Biology. 11:4.8:4.8.1–4.8.9.
- Published Online: 1 AUG 2001
- Published Print: JUL 2001
This unit describes newly developed methods that allow the examination of living cells by time-lapse analysis with the subsequent identification of the just-observed organelle under an electron microscope. To understand how such cellular functions, such as intracellular traffic, cytokinesis, and cell migration, are organized and executed in vivo, it is most useful to observe living cells in real time with the spatial resolution afforded by electron microscopy (EM). Most suitable for this is a conceptually simple, yet powerful, method called correlative video light/electron microscopy (CVLEM), by which observations of the in vivo dynamics and the ultrastructure of intracellular objects can indeed be combined to achieve the above-mentioned result. This unit describes this methodology, illustrates the type of questions that the CVLEM approach was designed to address, and discusses the expertise required for successful application of the technique.