UNIT 4.10 Fluorescent Speckle Microscopy (FSM) of Microtubules and Actin in Living Cells
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Waterman-Storer, C. 2002. Fluorescent Speckle Microscopy (FSM) of Microtubules and Actin in Living Cells. Current Protocols in Cell Biology. 13:4.10:4.10.1–4.10.26.
- Published Online: 1 FEB 2002
- Published Print: JAN 2002
Fluorescent speckle microscopy (FSM), a combination of conventional wide-field fluorescent light microscopy and digital imaging with a low-noise, charge-coupled device (CCD) camera, has been developed to allow visualization of assembly/disassembly dynamics, movement, and turnover of macromolecule assemblies in vivo and in vitro. FSM uses a low level of fluorescent subunits to avoid high background. This produces an image of speckled molecules that co-assemble with endogenous molecules and are followed to characterize dynamic events in living cells.