UNIT 4.16 Analyzing Real-Time Video Microscopy: The Dynamics and Geometry of Vesicles and Tubules in Endocytosis
Published Online: 1 JUN 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Hamilton, N., Kerr, M. C., Burrage, K. and Teasdale, R. D. 2007. Analyzing Real-Time Video Microscopy: The Dynamics and Geometry of Vesicles and Tubules in Endocytosis. Current Protocols in Cell Biology. 35:4.16:4.16.1–4.16.11.
- Published Online: 1 JUN 2007
- Published Print: JUN 2007
With the advent of live cell imaging microscopy, new types of mathematical analyses and measurements are possible. Many of the real-time movies of cellular processes are visually very compelling, but elementary analysis of changes over time of quantities such as surface area and volume often show that there is more to the data than meets the eye. This unit outlines a geometric modeling methodology and applies it to tubulation of vesicles during endocytosis. Using these principles, it has been possible to build better qualitative and quantitative understandings of the systems observed, as well as to make predictions about quantities such as ligand or solute concentration, vesicle pH, and membrane trafficked. The purpose is to outline a methodology for analyzing real-time movies that has led to a greater appreciation of the changes that are occurring during the time frame of the real-time video microscopy and how additional quantitative measurements allow for further hypotheses to be generated and tested.
- video microscopy;
- endosomal trafficking;
- membrane trafficking