UNIT 4.24 Fluorescence Correlation Spectroscopy in Living Cells: A Practical Approach
Published Online: 1 DEC 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Altan-Bonnet, N. and Altan-Bonnet, G. 2009. Fluorescence Correlation Spectroscopy in Living Cells: A Practical Approach. Current Protocols in Cell Biology. 45:4.24:4.24.1–4.24.14.
- Published Online: 1 DEC 2009
- Published Print: DEC 2009
Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion timescales and the total number of diffusing fluorescent molecules in the cell. A practical step-by-step approach to performing FCS with a commercial spectroscopy/microscopy system, the Zeiss Confocor 3, how to set up live-cell FCS experiments, acquire reliable data, and finally how to analyze the data acquired, are all described in this unit. Curr. Protoc. Cell Biol. 45:4.24.1-4.24.14. © 2009 by John Wiley & Sons, Inc.
- fluorescence correlation spectroscopy;
- live-cell imaging;
- green fluorescent protein;