Unit

UNIT 5.6 Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry

  1. Claire Delahunty,
  2. John R. Yates III

Published Online: 1 FEB 2003

DOI: 10.1002/0471143030.cb0505s17

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Delahunty, C. and Yates, J. R. 2003. Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry. Current Protocols in Cell Biology. 17:5.6:5.6.1–5.6.17.

Author Information

  1. The Scripps Research Institute, La Jolla, California

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: JAN 2003

Abstract

Liquid chromatography techniques have been successfully coupled with mass spectrometers to provide a robust method for the identification of proteins in mixtures. Chromatography can be performed in-line with the mass spectrometer and data acquisition can be directly interfaced with search algorithms for identification by correlation with databases.