UNIT 6.3 Detection and Quantitation of Radiolabeled Proteins in Gels and Blots
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Voytas, D. and Ke, N. 2001. Detection and Quantitation of Radiolabeled Proteins in Gels and Blots. Current Protocols in Cell Biology. 10:6.3:6.3.1–6.3.10.
- Published Online: 1 MAY 2001
- Published Print: APR 2001
Permanent records of the results of electrophoretic separations of radiolabeled proteins and membrane-bound proteins (and RNA and DNA) can be made using autoradiography, fluorography, and phosphor imaging. These images can subsequently be quantified using densitometry to obtain a relative measure of the amount of radioactivity in a sample. This unit also includes descriptions of image-enhancement techniques and guidelines for selection of appropriate recording media.