Unit

UNIT 6.3 Detection and Quantitation of Radiolabeled Proteins in Gels and Blots

  1. Daniel Voytas,
  2. Ning Ke

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0603s10

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Voytas, D. and Ke, N. 2001. Detection and Quantitation of Radiolabeled Proteins in Gels and Blots. Current Protocols in Cell Biology. 10:6.3:6.3.1–6.3.10.

Author Information

  1. Iowa State University, Ames, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

Abstract

Permanent records of the results of electrophoretic separations of radiolabeled proteins and membrane-bound proteins (and RNA and DNA) can be made using autoradiography, fluorography, and phosphor imaging. These images can subsequently be quantified using densitometry to obtain a relative measure of the amount of radioactivity in a sample. This unit also includes descriptions of image-enhancement techniques and guidelines for selection of appropriate recording media.