Unit

UNIT 6.7 Agarose Gel Electrophoresis of Proteins

  1. Dennis M. Krizek,
  2. Margaret E. Rick

Published Online: 1 AUG 2002

DOI: 10.1002/0471143030.cb0607s15

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Krizek, D. M. and Rick, M. E. 2002. Agarose Gel Electrophoresis of Proteins. Current Protocols in Cell Biology. 15:6.7:6.7.1–6.7.13.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUL 2002

Abstract

This unit describes electrophoretic separation and identification of large proteins from a complex protein mixture. Agarose gel is utilized for the electrophoretic matrix, and detection of proteins is accomplished by transfer of the proteins to a membrane that is probed with specific antibodies and chemiluminescence reagents. Alternatively the protein can be detected in the gel using radiolabeled antibodies and autoradiography.