UNIT 6.7 Agarose Gel Electrophoresis of Proteins
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Krizek, D. M. and Rick, M. E. 2002. Agarose Gel Electrophoresis of Proteins. Current Protocols in Cell Biology. 15:6.7:6.7.1–6.7.13.
- Published Online: 1 AUG 2002
- Published Print: JUL 2002
This unit describes electrophoretic separation and identification of large proteins from a complex protein mixture. Agarose gel is utilized for the electrophoretic matrix, and detection of proteins is accomplished by transfer of the proteins to a membrane that is probed with specific antibodies and chemiluminescence reagents. Alternatively the protein can be detected in the gel using radiolabeled antibodies and autoradiography.