Unit

UNIT 6.8 Fluorescence Detection of Glycoproteins in Gels and on Electroblots

  1. Wayne F. Patton

Published Online: 1 NOV 2002

DOI: 10.1002/0471143030.cb0608s16

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Patton, W. F. 2002. Fluorescence Detection of Glycoproteins in Gels and on Electroblots. Current Protocols in Cell Biology. 16:6.8:6.8.1–6.8.15.

Author Information

  1. Molecular Probes, Inc., Eugene, Oregon

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: OCT 2002

Abstract

Glycosylation of proteins is a dynamic post-translational modification commonly found in eukaryotes, changing with development, differentiation, and disease state. Identification of glycoproteins is important for completely characterizing the population of proteins found at a given point in time. This unit contains protocols for identifying glycoproteins in general using a fluorescence based periodate/Schiff base method and those containing specific carbohydrates using a lectin-based fluorescent method.