UNIT 6.8 Fluorescence Detection of Glycoproteins in Gels and on Electroblots
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Patton, W. F. 2002. Fluorescence Detection of Glycoproteins in Gels and on Electroblots. Current Protocols in Cell Biology. 16:6.8:6.8.1–6.8.15.
- Published Online: 1 NOV 2002
- Published Print: OCT 2002
Glycosylation of proteins is a dynamic post-translational modification commonly found in eukaryotes, changing with development, differentiation, and disease state. Identification of glycoproteins is important for completely characterizing the population of proteins found at a given point in time. This unit contains protocols for identifying glycoproteins in general using a fluorescence based periodate/Schiff base method and those containing specific carbohydrates using a lectin-based fluorescent method.