UNIT 7.2 Immunoprecipitation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Bonifacino, J. S. and Dell'Angelica, E. C. 2001. Immunoprecipitation. Current Protocols in Cell Biology. 00:7.2:7.2.1–7.2.21.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
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Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody.