Unit

UNIT 7.2 Immunoprecipitation

  1. Juan S. Bonifacino,
  2. Esteban C. Dell'Angelica

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0702s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Bonifacino, J. S. and Dell'Angelica, E. C. 2001. Immunoprecipitation. Current Protocols in Cell Biology. 00:7.2:7.2.1–7.2.21.

Author Information

  1. National Institute of Child Health and Human Development, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody.