UNIT 7.4 Metabolic Labeling with Fatty Acids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Jackson, C. S. and Magee, A. I. 2001. Metabolic Labeling with Fatty Acids. Current Protocols in Cell Biology. 5:7.4:7.4.1–7.4.10.
- Published Online: 1 MAY 2001
- Published Print: JAN 2000
Covalent attachment of radiolabeled fatty acids (e.g., [3H]myristate or palmitate) is an alternative method for labeling proteins. This unit contains methods for biosynthetic labeling with fatty acids, analysis of the fatty acid linkage with protein, analysis of total protein-bound fatty acid level in cell extracts, and analysis of the identity of the bound fatty acid.