UNIT 7.6 Metabolic Labeling and Immunoprecipitation of Yeast Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Graham, T. R. 2001. Metabolic Labeling and Immunoprecipitation of Yeast Proteins. Current Protocols in Cell Biology. 6:7.6:7.6.1–7.6.9.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
The proteins of Saccharomyces cervsiae can be metabolically labeled, as described here, with 35methionine and 35cysteine or a hydrolysate of E. coli labeled with 35O42−. After the labeling, protocols are provided for the mechanical disruption of the yeast cells or conversion to spheroplasts, with subsequent lysis before immunoprecipitation of the proteins.