Unit

UNIT 7.7 Metabolic Labeling and Immunoprecipitation of Drosophila Proteins

  1. Harish Radhakrishna

Published Online: 1 NOV 2001

DOI: 10.1002/0471143030.cb0707s12

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Radhakrishna, H. 2001. Metabolic Labeling and Immunoprecipitation of Drosophila Proteins. Current Protocols in Cell Biology. 12:7.7:7.7.1–7.7.6.

Author Information

  1. Georgia Institute of Technology, Atlanta, Georgia

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001

Abstract

The genetics of Drosophila is a powerful tool in the analysis of mutants and mutant proteins. Cultures of cells derived from wild-type or mutant flies can be pulse labeled to biosynthetically label the proteins made by the cells. Immunoprecipitation (and subcellular fractionation) are used to characterize the expression of specific proteins.