UNIT 7.8 Metabolic Labeling of Glycoproteins with Radioactive Sugars
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Snider, M. D. 2002. Metabolic Labeling of Glycoproteins with Radioactive Sugars. Current Protocols in Cell Biology. 13:7.8:7.8.1–7.8.11.
- Published Online: 1 FEB 2002
- Published Print: JAN 2002
This unit describes methods for preparation of glycoproteins metabolically labeled with radioactive sugars, sulfate, and phosphate. Methods for liberation of both N- and O-linked glycans are also described. These protocols can be used to generate materials for characterization of glycoprotein glycans from cultured cells.