Unit

UNIT 7.8 Metabolic Labeling of Glycoproteins with Radioactive Sugars

  1. Martin D. Snider

Published Online: 1 FEB 2002

DOI: 10.1002/0471143030.cb0708s13

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Snider, M. D. 2002. Metabolic Labeling of Glycoproteins with Radioactive Sugars. Current Protocols in Cell Biology. 13:7.8:7.8.1–7.8.11.

Author Information

  1. Case Western Reserve University, Cleveland, Ohio

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: JAN 2002

Abstract

This unit describes methods for preparation of glycoproteins metabolically labeled with radioactive sugars, sulfate, and phosphate. Methods for liberation of both N- and O-linked glycans are also described. These protocols can be used to generate materials for characterization of glycoprotein glycans from cultured cells.